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fluorogenic proteasome substrate iii suc-llvy-amc  (Millipore)

 
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    Millipore fluorogenic proteasome substrate iii suc-llvy-amc
    Fluorogenic Proteasome Substrate Iii Suc Llvy Amc, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluorogenic proteasome substrate iii suc-llvy-amc/product/Millipore
    Average 90 stars, based on 1 article reviews
    fluorogenic proteasome substrate iii suc-llvy-amc - by Bioz Stars, 2026-02
    90/100 stars

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    fluorogenic proteasome substrate iii suc-llvy-amc  (Millipore)
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    Left panel: P4.R5 cells were pretreated with MG-132 (25 μM) or nothing for 5 hours. The cells were then washed and exposed to 150 μM <t>Suc-LLVY-AMC</t> (with or without 25 μM MG-132) immediately or after a 2- or 5-hour incubation. Right panel: P4.R5 cells were pretreated with bortezomib (1 μM) or nothing for 5 hours. The cells were then washed and exposed to 150 μM Suc-LLVY-AMC (with or without 1 μM bortezomib) immediately or after a 2- or 5-hour incubation. Measurements were taken every 3 minutes in a fluorometer; the cells were maintained at 37° C. Each point represents the average of 3 measurements (C.V. < 1% in all cases). “LLVY” is suc-LLVY-AMC.
    Fluorogenic Proteasome Substrate Iii Suc Leu Leu Val Tyr Amc (Suc Llvy Amc, supplied by Enzo Biochem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Enzo Biochem fluorogenic proteasome substrate iii (suc-llvy-amc
    Left panel: P4.R5 cells were pretreated with MG-132 (25 μM) or nothing for 5 hours. The cells were then washed and exposed to 150 μM <t>Suc-LLVY-AMC</t> (with or without 25 μM MG-132) immediately or after a 2- or 5-hour incubation. Right panel: P4.R5 cells were pretreated with bortezomib (1 μM) or nothing for 5 hours. The cells were then washed and exposed to 150 μM Suc-LLVY-AMC (with or without 1 μM bortezomib) immediately or after a 2- or 5-hour incubation. Measurements were taken every 3 minutes in a fluorometer; the cells were maintained at 37° C. Each point represents the average of 3 measurements (C.V. < 1% in all cases). “LLVY” is suc-LLVY-AMC.
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    Left panel: P4.R5 cells were pretreated with MG-132 (25 μM) or nothing for 5 hours. The cells were then washed and exposed to 150 μM Suc-LLVY-AMC (with or without 25 μM MG-132) immediately or after a 2- or 5-hour incubation. Right panel: P4.R5 cells were pretreated with bortezomib (1 μM) or nothing for 5 hours. The cells were then washed and exposed to 150 μM Suc-LLVY-AMC (with or without 1 μM bortezomib) immediately or after a 2- or 5-hour incubation. Measurements were taken every 3 minutes in a fluorometer; the cells were maintained at 37° C. Each point represents the average of 3 measurements (C.V. < 1% in all cases). “LLVY” is suc-LLVY-AMC.

    Journal:

    Article Title: Evidence Against a Direct Antiviral Activity of the Proteasome during the Early Steps of HIV-1 Replication

    doi: 10.1016/j.virol.2007.01.018

    Figure Lengend Snippet: Left panel: P4.R5 cells were pretreated with MG-132 (25 μM) or nothing for 5 hours. The cells were then washed and exposed to 150 μM Suc-LLVY-AMC (with or without 25 μM MG-132) immediately or after a 2- or 5-hour incubation. Right panel: P4.R5 cells were pretreated with bortezomib (1 μM) or nothing for 5 hours. The cells were then washed and exposed to 150 μM Suc-LLVY-AMC (with or without 1 μM bortezomib) immediately or after a 2- or 5-hour incubation. Measurements were taken every 3 minutes in a fluorometer; the cells were maintained at 37° C. Each point represents the average of 3 measurements (C.V. < 1% in all cases). “LLVY” is suc-LLVY-AMC.

    Article Snippet: Fluorogenic Proteasome Substrate III Suc-Leu-Leu-Val-Tyr-AMC (Suc-LLVY-AMC) was purchased from Axxora (#BST-S-280) and resuspended in DMSO.

    Techniques: Incubation